Image MIME Reference
PCO-B16
PCO B16 Image
image/vnd.pco.b16
VS
RealNetworks RealFlash
image/vnd.rn-realflash
RealFlash
A complete technical comparison of PCO B16 Image and RealNetworks RealFlash — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
Equal browser support
Feature Support
| Feature | PCO-B16 | RealFlash |
|---|---|---|
| Transparency (Alpha) | ✕ No | Yes |
| Animation Support | ✕ No | Yes |
| Progressive Loading | ✕ No | Yes |
| HDR Support | ✕ No | ✕ No |
| EXIF Metadata | ✕ No | ✕ No |
| ICC Color Profile | ✕ No | ✕ No |
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
RealNetworks RealFlash
image/vnd.rn-realflash
Extension
Container
RealMedia / SWF
Compression
Vector (Flash) / RealAudio Compression
Algorithm
Macromedia Vector CompressionDeflate (Zlib)
Color Depth
8-bit, 24-bit, 32-bit
Developed by
RealNetworks & Macromedia
Released
1997
Magic Bytes
46 57 53 (FWS - Standard Flash) or 2E 52 4D 46 (.RMF - RealMedia Container)
Browser Support Comparison
| Browser | PCO-B16 | RealFlash |
|---|---|---|
Chrome
|
✕ | ✕ |
Firefox
|
✕ | ✕ |
Safari
|
✕ | ✕ |
Edge
|
✕ | ✕ |
IE (Legacy)
|
✕ | ✕ |
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
RealFlash
RealFlash Strengths
- Allowed complex, interactive vector animations to stream smoothly over 28.8k dial-up modems
- Perfectly synchronized streaming audio with timeline-based animations, which was revolutionary in 1997
Limitations
- Heavily reliant on proprietary browser plugins (RealPlayer), which were notoriously bloated
- Completely obsolete and unsupported on the modern web
- Tightly coupled server-client architecture required expensive RealServer licenses to host
When to choose which format
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry
RealFlash
Use RealFlash when…
- Historical Dial-up Web Animation
- Late-1990s Interactive Music Videos
- Streaming Vector Graphics