Image MIME Reference
AVCS
AVC Image Sequence (HEIF Container)
image/avcs
VS
PCO B16 Image
image/vnd.pco.b16
PCO-B16
A complete technical comparison of AVC Image Sequence (HEIF Container) and PCO B16 Image — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
Equal browser support
Feature Support
| Feature | AVCS | PCO-B16 |
|---|---|---|
| Transparency (Alpha) | Yes | ✕ No |
| Animation Support | Yes | ✕ No |
| Progressive Loading | ✕ No | ✕ No |
| HDR Support | Yes | ✕ No |
| EXIF Metadata | Yes | ✕ No |
| ICC Color Profile | Yes | ✕ No |
AVC Image Sequence (HEIF Container)
image/avcs
Extension
.avcs.heifs
Container
HEIF / ISOBMFF (ISO/IEC 14496-12)
Compression
Lossy / Lossless
Algorithm
AVC (Advanced Video Coding / H.264)
Color Depth
8-bit, 10-bit, 12-bit
Developed by
Moving Picture Experts Group (MPEG) / ISO/IEC JTC 1
Released
2015
Magic Bytes
XX XX XX XX 66 74 79 70 61 76 63 73
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
Browser Support Comparison
| Browser | AVCS | PCO-B16 |
|---|---|---|
Chrome
|
✕ | ✕ |
Firefox
|
✕ | ✕ |
Safari
|
✕ | ✕ |
Edge
|
✕ | ✕ |
IE (Legacy)
|
✕ | ✕ |
AVCS
AVCS Strengths
- Efficiently stores bursts of images or animations utilizing inter-frame compression
- Leverages the highly compatible and widely hardware-accelerated AVC (H.264) codec
- Robust HEIF container supports extensive metadata, alpha channels, and depth maps
Limitations
- Zero native support in web browsers
- Largely superseded in efficiency by newer sequence formats like HEICS (HEVC) or Animated AVIF (AV1)
- Complicated container structure requires specialized parsing libraries
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
When to choose which format
AVCS
Use AVCS when…
- Burst Photography
- Short Animations
- Camera Capture
- Archival Storage
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry