Image MIME Reference
PCO-B16
PCO B16 Image
image/vnd.pco.b16
VS
Portable Network Graphics (Legacy MIME)
image/x-png
PNG
A complete technical comparison of PCO B16 Image and Portable Network Graphics (Legacy MIME) — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
PNG wins on browser support
Feature Support
| Feature | PCO-B16 | PNG |
|---|---|---|
| Transparency (Alpha) | ✕ No | Yes |
| Animation Support | ✕ No | ✕ No |
| Progressive Loading | ✕ No | Yes |
| HDR Support | ✕ No | Yes |
| EXIF Metadata | ✕ No | Yes |
| ICC Color Profile | ✕ No | Yes |
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
Portable Network Graphics (Legacy MIME)
image/x-png
Extension
.png
Container
PNG Chunk Structure (IHDR, IDAT, IEND)
Compression
Deflate (Lossless)
Algorithm
Deflate (LZ77)
Color Depth
1-bit to 16-bit (Grayscale), 8-bit (Indexed Palette), 24-bit (True Color), 32-bit (True Color with Alpha), 48-bit (High Color), 64-bit (High Color with Alpha)
Developed by
PNG Development Group
Released
1996
Magic Bytes
89 50 4E 47 0D 0A 1A 0A
Browser Support Comparison
| Browser | PCO-B16 | PNG |
|---|---|---|
Chrome
|
✕ |
✓ 1
|
Firefox
|
✕ |
✓ 1
|
Safari
|
✕ |
✓ 1
|
Edge
|
✕ |
✓ 12
|
IE (Legacy)
|
✕ |
✓
|
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
PNG
PNG Strengths
- The underlying format provides flawless, lossless compression and perfect 8-bit alpha channel transparency
- Universally supported by every modern operating system, image editor, and web browser
Limitations
- The 'image/x-png' string is an obsolete vendor quirk that forces backend developers to write redundant normalization logic in file upload scripts
- If left unnormalized, strict MIME validators or content security policies on modern systems may incorrectly reject valid PNG files
When to choose which format
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry
PNG
Use PNG when…
- Legacy Internet Explorer File Uploads
- Older ASP/PHP Web Applications
- Lossless Web Graphics