Image MIME Reference
ICO
Windows Icon (Legacy MIME)
image/x-icon
VS
PCO B16 Image
image/vnd.pco.b16
PCO-B16
A complete technical comparison of Windows Icon (Legacy MIME) and PCO B16 Image — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
ICO wins on browser support
Feature Support
| Feature | ICO | PCO-B16 |
|---|---|---|
| Transparency (Alpha) | Yes | ✕ No |
| Animation Support | ✕ No | ✕ No |
| Progressive Loading | ✕ No | ✕ No |
| HDR Support | ✕ No | ✕ No |
| EXIF Metadata | ✕ No | ✕ No |
| ICC Color Profile | ✕ No | ✕ No |
Windows Icon (Legacy MIME)
image/x-icon
Extension
.ico
Container
ICO Directory Structure
Compression
Uncompressed (DIB/BMP) / Deflate (Embedded PNG)
Algorithm
NoneDeflate (via PNG)
Color Depth
1-bit, 4-bit, 8-bit, 24-bit (True Color), 32-bit (RGBA)
Developed by
Microsoft Corporation
Released
1985
Magic Bytes
00 00 01 00
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
Browser Support Comparison
| Browser | ICO | PCO-B16 |
|---|---|---|
Chrome
|
✓ 1
|
✕ |
Firefox
|
✓ 1
|
✕ |
Safari
|
✓ 1
|
✕ |
Edge
|
✓ 12
|
✕ |
IE (Legacy)
|
✓
|
✕ |
ICO
ICO Strengths
- Universally supported by every web browser in existence for website favicons
- Packs multiple resolutions into a single file, eliminating the need to serve different files for the taskbar vs. the desktop
- Modern support for embedded PNGs (since Windows Vista) allows for excellent 32-bit transparency (alpha channels) and drastically reduced file sizes
Limitations
- Internal file structure is convoluted due to decades of legacy cruft, such as the requirement for BMP data to lack a standard file header but double the height property for an archaic XOR/AND mask
- Larger file size compared to a single optimized PNG or SVG, as it literally stores the same image multiple times at different scales
- The MIME type landscape is highly fragmented (x-icon vs. vnd.microsoft.icon), causing occasional caching or strict-MIME validation errors on restrictive servers
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
When to choose which format
ICO
Use ICO when…
- Website Favicons (favicon.ico)
- Windows Application Executable Icons (.exe)
- Desktop Shortcut Icons
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry