Image MIME Reference
HSJ2
JPEG 2000 Image Sequence (Obsolete)
image/hsj2
VS
PCO B16 Image
image/vnd.pco.b16
PCO-B16
A complete technical comparison of JPEG 2000 Image Sequence (Obsolete) and PCO B16 Image — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
Equal browser support
Feature Support
| Feature | HSJ2 | PCO-B16 |
|---|---|---|
| Transparency (Alpha) | Yes | ✕ No |
| Animation Support | Yes | ✕ No |
| Progressive Loading | Yes | ✕ No |
| HDR Support | Yes | ✕ No |
| EXIF Metadata | Yes | ✕ No |
| ICC Color Profile | Yes | ✕ No |
JPEG 2000 Image Sequence (Obsolete)
image/hsj2
Extension
.hsj2
Container
HEIF / ISOBMFF (ISO/IEC 15444-16 Obsolete)
Compression
Lossy / Lossless
Algorithm
Motion JPEG 2000 (ISO/IEC 15444-3)
Color Depth
8-bit, 10-bit, 12-bit, 16-bit
Developed by
ISO/IEC JTC 1 / ITU-T
Released
2019
Magic Bytes
XX XX XX XX 66 74 79 70 68 73 6A 32
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
Browser Support Comparison
| Browser | HSJ2 | PCO-B16 |
|---|---|---|
Chrome
|
✕ | ✕ |
Firefox
|
✕ | ✕ |
Safari
|
✕ | ✕ |
Edge
|
✕ | ✕ |
IE (Legacy)
|
✕ | ✕ |
HSJ2
HSJ2 Strengths
- Historically attempted to combine Motion JPEG 2000 sequences with the flexible metadata capabilities of the HEIF container
- Supported mathematically lossless compression and scalable wavelet decoding
Limitations
- Officially deprecated by the standardization body (ISO/IEC JTC 1)
- Completely superseded by image/hej2k, rendering hsj2 files obsolete for any new development or archiving
- Zero web browser compatibility and negligible modern software support
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
When to choose which format
HSJ2
Use HSJ2 when…
- Legacy Archival
- Deprecated Workflows
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry