Image MIME Reference
HEIF-Sequence
High Efficiency Image File Format Sequence
image/heif-sequence
VS
PCO B16 Image
image/vnd.pco.b16
PCO-B16
A complete technical comparison of High Efficiency Image File Format Sequence and PCO B16 Image — covering compression, feature support, browser compatibility, magic bytes, and when to choose each format.
Equal browser support
Feature Support
| Feature | HEIF-Sequence | PCO-B16 |
|---|---|---|
| Transparency (Alpha) | Yes | ✕ No |
| Animation Support | Yes | ✕ No |
| Progressive Loading | ✕ No | ✕ No |
| HDR Support | Yes | ✕ No |
| EXIF Metadata | Yes | ✕ No |
| ICC Color Profile | Yes | ✕ No |
High Efficiency Image File Format Sequence
image/heif-sequence
Extension
.heifs.heif.hif
Container
HEIF / ISOBMFF (ISO/IEC 14496-12)
Compression
Lossy / Lossless
Algorithm
Codec Agnostic (HEVC, AVC, JPEG, AV1)
Color Depth
8-bit, 10-bit, 12-bit, 16-bit
Developed by
Moving Picture Experts Group (MPEG)
Released
2015
Magic Bytes
XX XX XX XX 66 74 79 70 6D 73 66 31
PCO B16 Image
image/vnd.pco.b16
Extension
.b16
Container
PCO B16 Binary Container
Compression
Uncompressed (Raw Sensor Data)
Algorithm
None
Color Depth
16-bit (Monochrome or Bayer Raw Color)
Developed by
PCO AG (Excelitas PCO GmbH)
Released
Early 2000s
Magic Bytes
50 43 4F 2D
Browser Support Comparison
| Browser | HEIF-Sequence | PCO-B16 |
|---|---|---|
Chrome
|
✕ | ✕ |
Firefox
|
✕ | ✕ |
Safari
|
✕ | ✕ |
Edge
|
✕ | ✕ |
IE (Legacy)
|
✕ | ✕ |
HEIF-Sequence
HEIF-Sequence Strengths
- Codec-agnostic container allows for extreme flexibility when storing multi-frame photographic data
- Maintains the space-saving benefits of ISOBMFF shared frame data blocks
- Excellent for archiving computational photography workloads like exposure brackets or focal stacks
Limitations
- Codec ambiguity means a decoder might be able to parse the container but fail to decode the underlying pixels
- Zero native web browser support
- Complex container structure requires specialized parsing libraries to extract individual frames
PCO-B16
PCO-B16 Strengths
- Preserves exact, uncompressed 16-bit raw sensor data, which is critically important for accurate scientific measurements, fluorescence microscopy, and photometry
- The simple, linear struct header is highly predictable and easy to parse in custom Python, MATLAB, or C++ scripts for researchers building bespoke pipelines
Limitations
- Generates massive file sizes due to the complete lack of image compression [1.3.1]
- Completely unsupported by standard operating systems, web browsers, and consumer image editing suites
- Strictly tied to the hardware and software ecosystems of a single camera manufacturer
When to choose which format
HEIF-Sequence
Use HEIF-Sequence when…
- Burst Photography
- Focal Stacking
- Exposure Bracketing
- Time-Lapse Data
PCO-B16
Use PCO-B16 when…
- Microscopy and Life Sciences
- High-Speed Camera Recording
- Machine Vision and Industrial Inspection
- Scientific Photometry